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Investigation of
Lepechinia graveolens for its antioxidant activity and phenolic
composition.
Parejo I, Caprai E, Bastida J,
Viladomat F, Jauregui O, Codina C.
Departament de Productes
Naturals, Biologia Vegetal i Edafologia, Facultat de Farmacia,
Universitat de Barcelona, Av. Joan XXIII s/n, 08028 Barcelona,
Catalonia, Spain.
A bioguided separation of Lepechinia graveolens
(Reg.) Epling. (Lamiaceae) for antioxidant activity was carried out. The
ethyl acetate fraction (EAF) from the methanolic extract was
chromatographed on Sephadex LH-20 affording five active fractions. The
radical scavenging activity of each fraction, as well as that of the
isolated compounds, was tested using three different methods. The major
isolated antioxidant compounds were identified as
luteolin-7-O-glucuronide, rosmarinic acid, and rosmarinic acid methyl
ester by means of [Formula: see text] and [Formula: see text] NMR and
mass spectrometry. Twenty other minor phenolic compounds were determined
by liquid chromatography-negative electrospray ionization tandem mass
spectrometry. They included hydroxycinnamic acid derivatives, flavonoids
and phenolic diterpenes. The major phenolic compound was found to be
rosmarinic acid, which was quantitatively determined by high-performance
liquid chromatography (HPLC)-diode array detector (DAD). The content of
rosmarinic acid was 2.8% (referred to dry weight), this amount being
around 30% of the total phenolic content (TPH) of the crude extract. It
can be concluded that the antioxidant activity of Lepechinia graveolens
was mainly due to rosmarinic acid, which allows this plant species to be
considered as a potential new source of this well known natural
antioxidant.
PMID: 15261980 [PubMed - in process]
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